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Generation and characterisation of CD1d tetramer produced by a lentiviral expression system.

Abstract:

The alpha-galactosylceramide (alphaGalCer)-loaded CD1d tetramer remains the most powerful tool in identifying natural killer T (NKT) cells, a subpopulation of T cells that express an unusual semi-invariant T cell antigen receptor, and mediate a variety of proinflammatory and immunoregulatory functions. The difficulty of generating large amounts of the alphaGalCer-CD1d tetramer has limited its availability and consequently hampered the study of NKT cells. In this report, we used a lentiviral s...

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Publication status:
Published

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Publisher copy:
10.1016/j.jim.2007.10.018

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Experimental Medicine Division,
Screaton, GR More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Experimental Medicine Division,
Journal:
Journal of immunological methods
Volume:
330
Issue:
1-2
Pages:
57-63
Publication date:
2008-01-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:f7d2fd83-907f-4e7a-867c-65ac36863d22
Source identifiers:
34073
Local pid:
pubs:34073

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