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Journal article

Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase.

Abstract:

FAD synthetase (FADS) (EC 2.7.7.2) is a key enzyme in the metabolic pathway that converts riboflavin into the redox cofactor FAD. Two hypothetical human FADSs, which are the products of FLAD1 gene, were over-expressed in Escherichia coli and identified by ESI-MS/MS. Isoform 1 was over-expressed as a T7-tagged protein which had a molecular mass of 63kDa on SDS-PAGE. Isoform 2 was over-expressed as a 6-His-tagged fusion protein, carrying an extra 84 amino acids at the N-terminal with an apparen...

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Publication status:
Published

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Publisher copy:
10.1016/j.bbrc.2006.04.003

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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Biochemical and biophysical research communications More from this journal
Volume:
344
Issue:
3
Pages:
1008-1016
Publication date:
2006-06-01
DOI:
EISSN:
1090-2104
ISSN:
0006-291X
Language:
English
Keywords:
Pubs id:
pubs:229429
UUID:
uuid:f78406d7-b22c-4760-bc8d-ef23e3edb23f
Local pid:
pubs:229429
Source identifiers:
229429
Deposit date:
2013-11-16

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