Mosaic HIV-1 Gag antigens can be processed and presented to human HIV-specific CD8+ T cells.

Ndhlovu, Z; Piechocka-Trocha, A; Vine, S; McMullen, A; Koofhethile, K; Goulder, P; Ndung'u, T; Barouch, D; Walker, B

Journal article

Mosaic HIV-1 Gag antigens can be processed and presented to human HIV-specific CD8+ T cells.

Abstract:: Polyvalent mosaic HIV immunogens offer a potential solution for generating vaccines that can elicit immune responses against genetically diverse viruses. However, it is unclear whether key T cell epitopes can be processed and presented from these synthetic Ags and recognized by epitope-specific human T cells. In this study, we tested the ability of mosaic HIV immunogens expressed by recombinant, replication-incompetent adenovirus serotype 26 vectors to process and present major HIV clade B and clade C CD8 T cell epitopes in human cells. A bivalent mosaic vaccine expressing HIV Gag sequences was used to transduce PBMCs from 12 HIV-1-infected individuals from the United States and 10 HIV-1-infected individuals from South Africa; intracellular cytokine staining, together with tetramer staining, was used to assess the ability of mosaic Gag Ags to stimulate pre-existing memory responses compared with natural clade B and C vectors. Mosaic Gag Ags expressed all eight clade B epitopes tested in 12 United States subjects and all 5 clade C epitopes tested in 10 South African subjects. Overall, the magnitude of cytokine production induced by stimulation with mosaic Ags was comparable to clade B and clade C Ags tested, but the mosaic Ags elicited greater cross-clade recognition. Additionally, mosaic Ags induced HIV-specific CD4 T cell responses. Our studies demonstrate that mosaic Ags express major clade B and clade C viral T cell epitopes in human cells, as well as support the evaluation of mosaic HIV-1 vaccines in humans.

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APA Style

Ndhlovu, Z., Piechocka-Trocha, A., Vine, S., McMullen, A., Koofhethile, K., Goulder, P., Ndung'u, T., Barouch, D., & Walker, B. (2011). Mosaic HIV-1 Gag antigens can be processed and presented to human HIV-specific CD8+ T cells. Journal of Immunology, 186(12), 6914–6924.

MLA Style

Ndhlovu, Z, et al. “Mosaic HIV-1 Gag Antigens Can Be Processed and Presented to Human HIV-Specific CD8+ T Cells.” Journal of Immunology, vol. 186, no. 12, 2011, pp. 6914–24.

Chicago Style

Ndhlovu, Z, A Piechocka-Trocha, S Vine, et al. 2011. “Mosaic HIV-1 Gag Antigens Can Be Processed and Presented to Human HIV-Specific CD8+ T Cells.” Journal of Immunology 186 (12): 6914–24.
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