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Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.

Abstract:

The fluorescent-protein based fluorescence resonance energy transfer (FRET) approach is a powerful method for quantifying protein-protein interactions in living cells, especially when combined with fluorescence lifetime imaging microscopy (FLIM). To compare the performance of different FRET couples for FRET-FLIM experiments, we first tested enhanced green fluorescent protein (EGFP) linked to different red acceptors (mRFP1-EGFP, mStrawberry-EGFP, HaloTag (TMR)-EGFP, and mCherry-EGFP). We obtai...

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Publisher copy:
10.1016/j.bpj.2009.07.044

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Journal:
Biophysical journal
Volume:
97
Issue:
8
Pages:
2368-2376
Publication date:
2009-10-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:f7535d49-3478-45a6-a4b1-51cbbc72bc06
Source identifiers:
394788
Local pid:
pubs:394788

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