Journal article
Spermidine restores dysregulated autophagy and polyamine synthesis in aged and osteoarthritic chondrocytes via EP300
- Abstract:
- Ageing is the primary risk factor for osteoarthritis (OA). A decline in the ageing-associated process of autophagy is suggested as a potential contributor to OA development. Polyamines such as spermidine decrease during ageing, contributing to impaired autophagy and reduced cellular function. However, the role of polyamines and their effect on the regulatory mechanism governing autophagy in aged and arthritic cartilage tissue has not been established. Elucidating if polyamine regulation of autophagy is impaired during ageing and OA in chondrocytes may lead to improved treatment approaches to protect against cartilage degradation. Our results indicate that polyamine synthesis was decreased in aged and OA cartilage, along with reduced autophagy activity, evidenced by decreased autophagyrelated gene and protein expression and autophagosome formation. Importantly, spermidine treatment increased the expression of the acetyltransferase EP300, which binds to crucial autophagy proteins, Beclin1 and LC3, and elevates chondrocyte autophagy. Our data indicate spermidine prevents the ageing- and OA-related decrease in autophagy and may protect against OA development.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, pdf, 1.7MB, Terms of use)
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- Publisher copy:
- 10.1038/s12276-018-0149-3
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Experimental and Molecular Medicine EMM More from this journal
- Volume:
- 50
- Issue:
- 6
- Pages:
- 123
- Publication date:
- 2018-09-19
- Acceptance date:
- 2018-06-25
- DOI:
- EISSN:
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2092-6413
- ISSN:
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1226-3613
- Pubs id:
-
pubs:918787
- UUID:
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uuid:f73c04d0-051f-489b-866a-bf524b284f0d
- Local pid:
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pubs:918787
- Deposit date:
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2018-09-12
Terms of use
- Copyright holder:
- Sacitharan et al
- Copyright date:
- 2018
- Notes:
- © The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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