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Immunohistological screening in the selection of monoclonal antibodies: the use of isotype-specific antiglobulins.

Abstract:
This paper describes the use of the immunoperoxidase technique for the screening of rat hybridoma culture supernatants on tissue sections. By combining the avidin-biotin system with mouse monoclonal antibodies specific to different rat immunoglobulin isotypes, it is possible to resolve the specificity patterns of complex mixtures of monoclonal antibodies from uncloned culture wells. This strategy is particularly useful in the derivation of monoclonal antibodies to cell surface antigens.
Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
Journal of immunological methods
Volume:
69
Issue:
2
Pages:
207-214
Publication date:
1984-04-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:f73737bd-ae6a-4067-891d-c9da2a70dbc3
Source identifiers:
12105
Local pid:
pubs:12105

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