YAC clone contigs surrounding the Zfx and Pola loci on the mouse X chromosome.

Journal article

Pulsed-field mapping of a number of DNA markers in the Pola-Zfx region of the mouse X chromosome has established a genomic restriction map extending over 1.4 Mb. A number of YAC clones from the Pola-Zfx region have been isolated from three mouse YAC libraries--first, a mouse C57BL/10 partial R1 YAC library constructed in a yeast strain carrying a rad52 mutation (Chartier et al. (1992) Nature Genetics 1: 132-136); second, a mouse C3H partial R1 library (Larin et al. (1991) Proc. Natl. Acad. Sci. USA 88: 4123-4127); and third, a mouse C57BL/6 partial R1 library (Burke et al. (1991) Mamm. Genome 1:65). Six YAC clones encompass the Zfx-Pola region, confirming the linkage of the Pola and Zfx loci and establishing a physical map order in this region of cen-Pola-DXCrc140-DXCrc57-Zfx-tel. The close linkage of Pola and Zfx in the mouse genome suggests that the POLA and ZFX loci must also be closely linked on the human X chromosome.

Authors: Hamvas, R; Larin, Z; Brockdorff, N; Rastan, S; Lehrach, H

• Publication status: Published
• Journal: Genomics
• Volume: 17
• Issue: 1
• Pages: 52-58
• Publication date: 1993-07-01
• DOI: 10.1006/geno.1993.1282
• EISSN: 1089-8646
• ISSN: 0888-7543
• Language: English
• Keywords: Molecular Sequence Data; X Chromosome; Animals; DNA-Binding Proteins; Genome, Human; Female; Kruppel-Like Transcription Factors; Gene Library; Restriction Mapping; Chromosome Walking; Chromosomes, Fungal; Genetic Markers; Mice, Inbred C3H; Humans; Base Sequence; Male; Mice; Mice, Inbred C57BL; Electrophoresis, Gel, Pulsed-Field; Transcription Factors