Journal article
Automation of large scale transient protein expression in mammalian cells
- Abstract:
-
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline. Transient transfections are a fast, cost-effective solution, but require skilled cell culture scientists, making man-power a limiting factor in a setting where numerous samples are processed in parallel. Here we report a strategy employing a customised CompacT SelecT cell culture robot allowing the large-scale expres...
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- Publisher copy:
- 10.1016/j.jsb.2011.04.017
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Authors
Bibliographic Details
- Journal:
- Journal of Structural Biology
- Volume:
- 175
- Issue:
- 2
- Pages:
- 209-215
- Publication date:
- 2011-08-01
- DOI:
- EISSN:
-
1095-8657
- ISSN:
-
1047-8477
- Source identifiers:
-
166765
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:166765
- UUID:
-
uuid:f6c55b16-57e8-4bdf-bd6c-028ff51e4849
- Local pid:
- pubs:166765
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2011
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