- Abstract:
-
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline. Transient transfections are a fast, cost-effective solution, but require skilled cell culture scientists, making man-power a limiting factor in a setting where numerous samples are processed in parallel. Here we report a strategy employing a customised CompacT SelecT cell culture robot allowing the large-scale expres...
Expand abstract - Publisher copy:
- 10.1016/j.jsb.2011.04.017
-
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- Journal:
- Journal of Structural Biology
- Volume:
- 175
- Issue:
- 2
- Pages:
- 209-215
- Publication date:
- 2011-08-05
- DOI:
- EISSN:
-
1095-8657
- ISSN:
-
1047-8477
- URN:
-
uuid:f6c55b16-57e8-4bdf-bd6c-028ff51e4849
- Source identifiers:
-
166765
- Local pid:
- pubs:166765
- Language:
- English
- Keywords:
- Copyright date:
- 2011
Journal article
Automation of large scale transient protein expression in mammalian cells
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