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Automation of large scale transient protein expression in mammalian cells

Abstract:

Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline. Transient transfections are a fast, cost-effective solution, but require skilled cell culture scientists, making man-power a limiting factor in a setting where numerous samples are processed in parallel. Here we report a strategy employing a customised CompacT SelecT cell culture robot allowing the large-scale expres...

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Publisher copy:
10.1016/j.jsb.2011.04.017

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Journal:
Journal of Structural Biology
Volume:
175
Issue:
2
Pages:
209-215
Publication date:
2011-08-05
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:f6c55b16-57e8-4bdf-bd6c-028ff51e4849
Source identifiers:
166765
Local pid:
pubs:166765

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