Amino acid sequence data of alpha-tubulin from myxamoebae of Physarum polycephalum.

Journal article

About 96% of the amino acid sequence of an alpha-tubulin from the slime mould Physarum polycephalum has been determined. Of 430 sequenced amino acids, 30 differ from the deduced amino acid sequence of a recently published alpha-tubulin complementary DNA from the plasmodial form of P. polycephalum. The myxamoebal alpha-tubulin differs from all other known alpha-tubulins in one of the last three C-terminal amino acids that are Gly-Glu-Tyr instead of the usual Glu-Glu-Tyr. These last three amino acids are preceded by 11 residues that appear to be particularly susceptible to mutation. No heterogeneity was found whilst sequencing the myxamoebal alpha-tubulin, indicating that only one type of alpha-tubulin is present in myxamoebae. This alpha-tubulin appears to be less conserved than the previously described plasmodial alpha-tubulin, supporting the hypothesis that the structural constraints on tubulin in axonemes have a significant effect on its rate of mutation.

Authors: Singhofer-Wowra, M; Little, M; Clayton, L; Dawson, P; Gull, K

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 192
• Issue: 4
• Pages: 919-924
• Publication date: 1986-12-01
• DOI: 10.1016/0022-2836(86)90037-9
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Chickens; Physarum; Tubulin; Sea Urchins; Swine; Animals; Rats; Humans; Amino Acid Sequence