X-ray crystallography of agonist/antagonist-bound receptors.

Journal article

Crystallographic analysis of the ligand-binding domains of nuclear hormone receptors (NR) has provided a unique insight into the molecular events that underlie the ligand-mediated control of their transcriptional activity. The technique relies on preparing milligram quantities of protein, growing three-dimensional crystals of the desired protein-ligand complex, collecting X-ray diffraction data, and subsequently interpreting the derived electron density maps to reveal the structure of the complex.

Authors: Pike, A

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 505
• Pages: 51-66
• Publication date: 2009-01-01
• ISSN: 1064-3745
• Language: English
• Subjects: methods; Crystallography, X-Ray; metabolism; Ligands; Protein Conformation; instrumentation; Crystallization; chemistry; Receptors, Cytoplasmic and Nuclear; Estrogen Receptor alpha