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Polymorphisms of transporter associated with antigen presentation, tumor necrosis factor-α and interleukin-10 and their implications for protection and susceptibility to severe forms of dengue fever in patients in Sri Lanka

Abstract:

Context: To date, a clear understanding of dengue disease pathogenesis remains elusive. Some infected individuals display no symptoms while others develop severe life-threatening forms of the disease. It is widely believed that host genetic factors influence dengue severity.

Aims: This study evaluates the relationship between certain polymorphisms and dengue severity in Sri Lankan patients.

Settings and Design: Polymorphism studies are carried out on genes for; transporter associated with antigen presentation (TAP), promoter of tumor necrosis factor-α (TNF-α), and promoter of interleukin-10 (IL-10). In other populations, TAP1 (333), TAP2 (379), TNF-α (−308), and IL-10 (−1082, −819, −592) have been associated with dengue and a number of different diseases. Data have not been collected previously for these polymorphisms for dengue patients in Sri Lanka.

Materials and Methods: The polymorphisms were typed by amplification refractory mutation system polymerase chain reaction in 107 dengue hemorrhagic fever (DHF) patients together with 62 healthy controls.

Statistical Analysis Used: Pearson's Chi-square contingency table analysis with Yates′ correction.

Results: Neither the TAP nor the IL-10 polymorphisms considered individually can define dengue disease outcome with regard to severity. However, the genotype combination, IL-10 (−592/−819/−1082) CCA/ATA was significantly associated with development of severe dengue in these patients, suggesting a risk factor to developing DHF. Also, identified is the genotype combination IL-10 (−592/−819/−1082) ATA/ATG which suggested a possibility for protection from DHF. The TNF-α (−308) GG genotype was also significantly associated with severe dengue, suggesting a significant risk factor.

Conclusions: The results reported here are specific to the Sri Lankan population. Comparisons with previous reports imply that data may vary from population to population.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.4103/0974-777x.170501

Authors

More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Inst of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Investigative Medicine
Role:
Author
ORCID:
0000-0002-3097-045X


Publisher:
Medknow Publications
Journal:
Journal of Global Infectious Diseases More from this journal
Volume:
7
Issue:
4
Pages:
157-164
Publication date:
2015-10-01
DOI:
EISSN:
0974-8245
ISSN:
0974-777X


Language:
English
Keywords:
Pubs id:
pubs:588679
UUID:
uuid:f65461d5-fc1b-4262-830f-92624f4171fc
Local pid:
pubs:588679
Source identifiers:
588679
Deposit date:
2016-03-30
ARK identifier:

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