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Journal article : Review

What can we learn about acid-base transporters in cancer from studying somatic mutations in their genes?

Abstract:

Acidosis is a chemical signature of the tumour microenvironment that challenges intracellular pH homeostasis. The orchestrated activity of acid-base transporters of the solute-linked carrier (SLC) family is critical for removing the end-products of fermentative metabolism (lactate/H+) and maintaining a favourably alkaline cytoplasm. Given the critical role of pH homeostasis in enabling cellular activities, mutations in relevant SLC genes may impact the oncogenic process, emerging as negatively or positively selected, or as driver or passenger mutations. To address this, we performed a pan-cancer analysis of The Cancer Genome Atlas simple nucleotide variation data for acid/base-transporting SLCs (ABT-SLCs). Somatic mutation patterns of monocarboxylate transporters (MCTs) were consistent with their proposed essentiality in facilitating lactate/H+ efflux. Among all cancers, tumours of uterine corpus endometrial cancer carried more ABT-SLC somatic mutations than expected from median tumour mutation burden. Among these, somatic mutations in SLC4A3 had features consistent with meaningful consequences on cellular fitness. Definitive evidence for ABT-SLCs as ‘cancer essential’ or ‘driver genes’ will have to consider microenvironmental context in genomic sequencing because bulk approaches are insensitive to pH heterogeneity within tumours. Moreover, genomic analyses must be validated with phenotypic outcomes (i.e. SLC-carried flux) to appreciate the opportunities for targeting acid-base transport in cancers.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/s00424-023-02876-y

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Oxford college:
Corpus Christi College
Role:
Author
ORCID:
0000-0002-9327-3168
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Oxford college:
Corpus Christi College
Role:
Author
ORCID:
0000-0002-9945-9473


Publisher:
Springer
Journal:
Pflügers Archiv European Journal of Physiology More from this journal
Volume:
476
Issue:
4
Pages:
673-688
Publication date:
2023-11-24
Acceptance date:
2023-10-30
DOI:
EISSN:
1432-2013
ISSN:
0031-6768
Pmid:
37999800


Language:
English
Keywords:
Subtype:
Review
Pubs id:
1571507
Local pid:
pubs:1571507
Deposit date:
2023-11-27

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