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Multiple displacement amplification for malaria parasite DNA.

Abstract:

Multiple displacement amplification (MDA) using Phi29 has proved to be an efficient, high-fidelity method for whole genome amplification in many organisms. This project was designed to evaluate this approach for use with the malaria parasite Plasmodium falciparum. In particular, we were concerned that the AT richness and presence of contaminating human DNA could limit efficiency of MDA in this system. We amplified 60 DNA samples using phi29 and scored 14 microsatellites, 9 single-nucleotide p...

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Publication status:
Published

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Publisher copy:
10.1645/ge-1706.1

Authors


Journal:
Journal of parasitology
Volume:
95
Issue:
1
Pages:
253-255
Publication date:
2009-02-01
DOI:
EISSN:
1937-2345
ISSN:
0022-3395
Source identifiers:
18098
Language:
English
Keywords:
Pubs id:
pubs:18098
UUID:
uuid:f6191aa3-1625-470c-87e0-0dff86a02791
Local pid:
pubs:18098
Deposit date:
2012-12-19

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