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Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells: a "one plasmid" mammalian two-hybrid system.

Abstract:

As a complement to the intracellular antibody capture method to isolate intracellular single domain antibody fragments (iDabs) from high diverse libraries, we describe here a simple mammalian two-hybrid (M2H) protocol using a "bait-prey hybrid single plasmid" to assess those interfering iDabs that will block protein-protein interactions of a target with its natural partner proteins. This rapid method identifies interfering iDabs in one step and improves the reproducibility of the results betw...

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Volume:
911
Pages:
175-182
Publication date:
2012-01-01
DOI:
URN:
uuid:f5ea8e1f-4b1f-4030-b28c-1fbf7a3fbf1d
Source identifiers:
353642
Local pid:
pubs:353642
ISBN:
9781617799679

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