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Nanoscale separation of molecular species based on their rotational mobility.

Abstract:
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
Publication status:
Published

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Publisher copy:
10.1364/oe.16.021093

Authors


Publisher:
Optical Society of American (OSA)
Journal:
Optics express More from this journal
Volume:
16
Issue:
25
Pages:
21093-21104
Publication date:
2008-12-01
DOI:
EISSN:
1094-4087
ISSN:
1094-4087
Language:
English
Keywords:
Pubs id:
pubs:222189
UUID:
uuid:f5e05e37-cd01-476d-be51-ec400fdafe68
Local pid:
pubs:222189
Source identifiers:
222189
Deposit date:
2012-12-19

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