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An investigation of N metabolism and pH regulation in Sphagnum using in vivo nuclear magnetic resonance and stable isotope mass spectrometry

Abstract:

Glutamine synthetase (GS) and glutamate dehydrogenase (GDH) are both present in Sphagnum and in principle both enzymes could be involved in the assimilation of ammonium. 15NH4+ application led to the formation γ- 15N-glutamine, followed by α-amino glutamate/glutamine as observed by in vivo 15N NMR. No assimilation was detected in the presence of the OS-specific inhibitor methionine sulfoximine. This labelling pattern suggests, that ammonium assimilation in Sphagnum is mediated solely by the G...

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Publication status:
Published

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Publisher copy:
10.1080/10256019808036355

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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Host title:
ISOTOPES IN ENVIRONMENTAL AND HEALTH STUDIES
Volume:
34
Issue:
1-2
Pages:
21-29
Publication date:
1998-01-01
DOI:
EISSN:
1477-2639
ISSN:
1025-6016
Keywords:
Pubs id:
pubs:36391
UUID:
uuid:f5ccce81-3686-4db5-97ed-41b8dc9f8157
Local pid:
pubs:36391
Source identifiers:
36391
Deposit date:
2012-12-19

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