An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism.

Journal article

In previous studies of antigen presentation through HLA-B27, we identified a healthy person whose lymphoblastoid cells do not present three B27-restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA-B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA-A3-B2702 haplotype. The original donor, NW, carries HLA-B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA-B8-restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3-B2702 haplotype, present the expected epitope after viral infection. Another B8-restricted epitope, from human immunodeficiency virus-gag, is presented equally well by both cell lines when infected with gag-vaccinia. This antigen processing phenotype does not correlate with any of the known human TAP-1 and TAP-2 polymorphisms.

Authors: Rowland-Jones, S; Powis, S; Sutton, J; Mockridge, I; Gotch, F

• Publication status: Published
• Journal: European journal of immunology
• Volume: 23
• Issue: 8
• Pages: 1999-2004
• Publication date: 1993-08-01
• DOI: 10.1002/eji.1830230841
• EISSN: 1521-4141
• ISSN: 0014-2980
• Language: English
• Keywords: Polymorphism, Genetic; Female; Viral Core Proteins; RNA-Binding Proteins; Nucleoproteins; HLA-B8 Antigen; Male; Histocompatibility Antigens Class II; Antigen-Presenting Cells; T-Lymphocytes, Cytotoxic; Humans; Carrier Proteins; ATP-Binding Cassette Transporters