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Orientation of retinal in bacteriorhodopsin as studied by cross-linking using a photosensitive analog of retinal.

Abstract:

The photosensitive m-diazirinophenyl analog of retinal (Fig. 1, II) bound to bacterio-opsin at Lys-216 and regenerated a chromophore with lambda max at 470 nm. Photolysis of the complex at 365 nm resulted in covalent cross-linking of the retinal analog to the bacterio-opsin in greater 30% yield. Investigation of the sites of cross-linking between the 3H-labeled retinal analog and the protein showed the peptide fragment (amino acid residues 190-248) to be the main radioactively labeled product...

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Publication status:
Published

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Authors


Radhakrishnan, R More by this author
Khorana, HG More by this author
Journal:
The Journal of biological chemistry
Volume:
257
Issue:
22
Pages:
13616-13623
Publication date:
1982-11-05
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:f5be2100-9ce0-4996-93bb-44fbef723efa
Source identifiers:
52411
Local pid:
pubs:52411

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