Orientation of retinal in bacteriorhodopsin as studied by cross-linking using a photosensitive analog of retinal.
The photosensitive m-diazirinophenyl analog of retinal (Fig. 1, II) bound to bacterio-opsin at Lys-216 and regenerated a chromophore with lambda max at 470 nm. Photolysis of the complex at 365 nm resulted in covalent cross-linking of the retinal analog to the bacterio-opsin in greater 30% yield. Investigation of the sites of cross-linking between the 3H-labeled retinal analog and the protein showed the peptide fragment (amino acid residues 190-248) to be the main radioactively labeled product...Expand abstract
- Publication status:
- The Journal of biological chemistry
- Publication date:
- Source identifiers:
- Local pid:
- Copyright date: