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A nano-electrospray investigation into the subunit exchange of small heat shock proteins

Abstract:
The use of nano electrospray ionization (ESI) data for the subunit exchange of small heat shock proteins (sHSP) is discussed. The sHSP are maintained intact through the mass spectrometer using appropriate solution conditions, and careful control of gas-phase parameters. The mixtures of HSP16.9 and 18.1 are examined, both after equilibration and in real-time immediately after mixing. A comparison with the spectra indicates that after equilibration, the distribution of the hetero-complexes is governed by the starting ratio of the two components.

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Pages:
437-438
Host title:
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication date:
2002-01-01
Source identifiers:
323808
Pubs id:
pubs:323808
UUID:
uuid:f5b28add-ca21-4a8d-b6ef-529d271de33f
Local pid:
pubs:323808
Deposit date:
2013-02-20

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