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Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.

Abstract:

Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) with photoswitching, the reversible activation and deactivation of fluorophores by light. By photoswitching, multiple donor-acceptor fluorophore pairs can be probed sequentially, thus allowing observation of multiple distances within a single immobilized molecule. Control of the photoinduced switching rates permits adjustment of the temporal resolution of switchable FRET over a wide range of times...

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Publication status:
Published

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Publisher copy:
10.1002/cphc.201000834

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Kapanidis, AN More by this author
Journal:
Chemphyschem : a European journal of chemical physics and physical chemistry
Volume:
12
Issue:
3
Pages:
571-579
Publication date:
2011-02-05
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:f59de41b-a099-45b9-badd-ecc4342d4cb8
Source identifiers:
115755
Local pid:
pubs:115755

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