Gain- and loss-of-function approaches in the chick embryo.

Journal article

The chicken embryo has been used as a classical embryological model for studying developmental events because of its ready availability, similarity to the human embryos, and amenability to embryological and surgical manipulations. With the arrival of the molecular era, however, avian embryos presented distinct experimental limitations, largely because of the difficulty of performing targeted mutagenesis or transgenic studies. However, in the last decade and a half, a number of new methods for...

Expand abstract

Authors: Sauka-Spengler, T; Barembaum, M

• Journal: Methods in cell biology
• Volume: 87
• Pages: 237-256
• Publication date: 2008-01-01
• DOI: 10.1016/s0091-679x(08)00212-4
• ISSN: 0091-679X
• Language: English
• Keywords: Retroviridae; Models, Biological; Animals; Electroporation; Ectoderm; Genes, Dominant; Gene Expression Regulation, Developmental; Heterozygote; Gene Targeting; Chick Embryo; RNA Interference; Neural Crest; Animals, Genetically Modified; Organ Specificity; Gene Transfer Techniques