Regulation of bim in glucocorticoid-mediated osteoblast apoptosis.

Journal article

Osteoblasts undergo apoptosis both in vitro and in vivo in response to high dose glucocorticoid (GC) treatment. However, the molecular mechanisms remain elusive, hindering the prevention and treatment of this side-effect. Apoptosis was induced by dexamethasone (Dex) in murine MBA-15.4 osteoblasts within 24-48 h of treatment. We found dose- and time-dependent upregulation of Bim protein, a pro-apoptotic Bcl-2 family member, with highest levels at 24-48 h for 1 microM Dex. This was also observe...

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Authors: Espina, B; Liang, M; Russell, R; Hulley, P

• Publication status: Published
• Journal: Journal of cellular physiology
• Volume: 215
• Issue: 2
• Pages: 488-496
• Publication date: 2008-05-01
• DOI: 10.1002/jcp.21335
• EISSN: 1097-4652
• ISSN: 0021-9541
• Language: English
• Keywords: Dose-Response Relationship, Drug; Culture Media, Serum-Free; Dexamethasone; Receptors, Glucocorticoid; Up-Regulation; Mifepristone; bcl-2-Associated X Protein; Membrane Proteins; Time Factors; Apoptosis; Stromal Cells; RNA, Small Interfering; Apoptosis Regulatory Proteins; Osteoblasts; Cells, Cultured; Cysteine Proteinase Inhibitors; Bone Marrow Cells; Glucocorticoids; Proto-Oncogene Proteins; Leupeptins; Extracellular Matrix; Humans; Cycloheximide; RNA, Messenger