Journal article

Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Abstract:: This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles.Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels.C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation.Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Barkalina, N., Jones, C., Townley, H., & Coward, K. (2015). Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro. Nanomedicine (London, England), 10(10), 1539–1553.

MLA Style

Barkalina, N, et al. “Functionalization of Mesoporous Silica Nanoparticles with a Cell-Penetrating Peptide to Target Mammalian Sperm in Vitro.” Nanomedicine (London, England), vol. 10, no. 10, 2015, pp. 1539–53.

Chicago Style

Barkalina, N, C Jones, H Townley, and K Coward. 2015. “Functionalization of Mesoporous Silica Nanoparticles with a Cell-Penetrating Peptide to Target Mammalian Sperm in Vitro.” Nanomedicine (London, England) 10 (10): 1539–53.
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Files:: Barkalina et al_031014.pdf

(Preview, Accepted manuscript, pdf, 1.2MB, Terms of use)

Publisher copy:: 10.2217/nnm.14.235

Authors

+ Barkalina, N More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Women's and Reproductive Health
Role:: Author

+ Jones, C More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Women's and Reproductive Health
Role:: Author

+ Townley, H More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Women's and Reproductive Health
Role:: Author

+ Coward, K More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Women's and Reproductive Health
Role:: Author

Publisher:: Future Medicine
Journal:: Nanomedicine (London, England) More from this journal
Volume:: 10
Issue:: 10
Pages:: 1539-1553
Publication date:: 2015-05-01
DOI:: 10.2217/nnm.14.235
EISSN:: 1748-6963
ISSN:: 1743-5889

Language:: English
Keywords:: mesoporous silica

delivery

functionalization

sperm

nanoparticles

cell-penetrating peptides
Pubs id:: pubs:524821
UUID:: uuid:f50c38e4-c1ae-4492-b86e-b0a5e9357a12
Local pid:: pubs:524821
Source identifiers:: 524821
Deposit date:: 2015-12-26
ARK identifier:: ark:/29072/ora_f50c38e4c1ae4492b86eb0a5e9357a12

Terms of use

Copyright holder:: Future Medicine
Copyright date:: 2015
Notes:: This is the accepted manuscript version of the article. The final version is available online from Future Medicine at: [doi: 10.2217/nnm.14.235]

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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