Identification of lymphoma-associated antigens using SEREX.

Journal article

Serological analysis of recombinant complementary deoxyribonucleic acid (cDNA) expression libraries (SEREX) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies that are naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognize bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full.

Authors: Liggins, A; Guinn, B; Banham, A

• Journal: Methods in molecular medicine
• Volume: 115
• Pages: 109-128
• Publication date: 2005-01-01
• DOI: 10.1385/1-59259-936-2:109
• EISSN: 1940-6037
• ISSN: 1543-1894
• Language: English
• Keywords: Autoantibodies; Gene Library; Antibodies, Neoplasm; Humans; Antigens, Neoplasm; Autoantigens; Tumor Markers, Biological; DNA, Complementary; Lymphoma