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Glycoprotein structural genomics: solving the glycosylation problem.

Abstract:

Glycoproteins present special problems for structural genomic analysis because they often require glycosylation in order to fold correctly, whereas their chemical and conformational heterogeneity generally inhibits crystallization. We show that the "glycosylation problem" can be solved by expressing glycoproteins transiently in mammalian cells in the presence of the N-glycosylation processing inhibitors, kifunensine or swainsonine. This allows the correct folding of the glycoproteins, but lea...

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Publication status:
Published

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Publisher copy:
10.1016/j.str.2007.01.011

Authors


Crispin, M More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Harvey, DJ More by this author
Nettleship, JE More by this author
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Journal:
Structure (London, England : 1993)
Volume:
15
Issue:
3
Pages:
267-273
Publication date:
2007-03-05
DOI:
EISSN:
1878-4186
ISSN:
0969-2126
URN:
uuid:f4c8bc88-6e17-4302-b13a-60d009394d70
Source identifiers:
21003
Local pid:
pubs:21003

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