Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells.

Journal article

Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous vein endothelial cells was upregulated between 2.5- and 40-fold by rIL-1, rTNF, LPS and rIFN gamma corresponding to up to 5 X 10(6) sites/cell. Endothelial cell ICAM-1 was a single band of 90 kD in SDS-PAGE. Purified endothelial cell ICAM-1 reconstituted into liposomes and bound to plastic was an excellent substrate for both JY B lymphoblastoid cell and T lymphoblast adhesion. Adhesion to endoth...

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Authors: Dustin, M; Springer, T

• Journal: Journal of cell biology
• Volume: 107
• Issue: 1
• Pages: 321-331
• Publication date: 1988-07-01
• DOI: 10.1083/jcb.107.1.321
• EISSN: 1540-8140
• ISSN: 0021-9525
• Language: English
• Keywords: B-Lymphocytes; Humans; Interferon-gamma; Antigens, Surface; Antibodies, Monoclonal; Lymphocyte Function-Associated Antigen-1; Lymphocytes; Saphenous Vein; Cell Line; Endothelium, Vascular; Lipopolysaccharides; Cells, Cultured; Fluorescent Antibody Technique; Cell Adhesion Molecules; Umbilical Veins; Tumor Necrosis Factor-alpha; Flow Cytometry; Recombinant Proteins; Escherichia coli; Cell Adhesion; T-Lymphocytes; Membrane Glycoproteins; Radioimmunoassay