Journal article icon

Journal article

Understanding the structural requirements for activators of the Kef bacterial potassium efflux system.

Abstract:

The potassium efflux system, Kef, protects bacteria against the detrimental effects of electrophilic compounds via acidification of the cytoplasm. Kef is inhibited by glutathione (GSH) but activated by glutathione-S-conjugates (GS-X) formed in the presence of electrophiles. GSH and GS-X bind to overlapping sites on Kef, which are located in a cytosolic regulatory domain. The central paradox of this activation mechanism is that GSH is abundant in cells (at concentrations of ∼10-20 mM), and thu...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1021/bi5001118

Authors


Ekkerman, S More by this author
Pliotas, C More by this author
Richard, M More by this author
Bartlett, W More by this author
Expand authors...
Publisher:
American Chemical Society
Journal:
Biochemistry
Volume:
53
Issue:
12
Pages:
1982-1992
Publication date:
2014-04-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:f42c9518-8f62-43e2-8d03-f4b6008836f3
Source identifiers:
458734
Local pid:
pubs:458734

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP