Functional consequences of a polymorphism affecting NF-kappaB p50-p50 binding to the TNF promoter region.

Journal article

Stimulation of the NF-kappaB pathway often causes p65-p50 and p50-p50 dimers to be simultaneously present in the cell nucleus. A natural polymorphism at nucleotide -863 in the human TNF promoter (encoding tumor necrosis factor [TNF]) region provides an opportunity to dissect the functional interaction of p65-p50 and p50-p50 at a single NF-kappaB binding site. We found that this site normally binds both p65-p50 and p50-p50, but a single base change specifically inhibits p50-p50 binding. Report...

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Authors: Udalova, I; Richardson, A; Denys, A; Smith, C; Ackerman, H

• Publication status: Published
• Journal: Molecular and cellular biology
• Volume: 20
• Issue: 24
• Pages: 9113-9119
• Publication date: 2000-12-01
• DOI: 10.1128/mcb.20.24.9113-9119.2000
• EISSN: 1098-5549
• ISSN: 0270-7306
• Language: English
• Keywords: COS Cells; NF-kappa B p50 Subunit; Dimerization; Cell Fractionation; Lipopolysaccharides; Binding Sites; Macrophages; Cloning, Molecular; Promoter Regions, Genetic; Tumor Necrosis Factor-alpha; Polymorphism, Genetic; Humans; Gene Expression Regulation; Transcriptional Activation; Monocytes; Animals; Adenoviridae; Cells, Cultured; Cell Line; Genes, Reporter; NF-kappa B