Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy.

Journal article

Fluorescence in situ hybridization on three-dimensionally preserved cells (3D-FISH) is an efficient tool to analyze the subcellular localization and spatial arrangement of targeted DNA sequences and RNA transcripts at the single cell level. 3D reconstructions from serial optical sections obtained by confocal laser scanning microscopy (CLSM) have long been considered the gold standard for 3D-FISH analyses. Recent super-resolution techniques circumvent the diffraction-limit of optical resolutio...

Expand abstract

Authors: Markaki, Y; Smeets, D; Cremer, M; Schermelleh, L

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 950
• Pages: 43-64
• Publication date: 2013-01-01
• DOI: 10.1007/978-1-62703-137-0_4
• EISSN: 1940-6029
• ISSN: 1064-3745
• Language: English
• Keywords: Cell Nucleus Shape; Uridine; Lighting; Imaging, Three-Dimensional; Staining and Labeling; Tissue Fixation; Mice; Fluorescent Antibody Technique; Indoles; DNA; Animals; Nucleic Acid Denaturation; In Situ Hybridization, Fluorescence; Microscopy