Journal article
Non‐equivalence in old‐ and new‐flagellum daughter cells of a proliferative division in Trypanosoma brucei
- Abstract:
- Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. Conversely, proliferative cell divisions produce two daughter cells, which look similar but are not identical. To examine in detail differences between the daughter cells of a proliferative division of procyclic T. brucei we used the recently identified constituents of the flagella connector. These segregate asymmetrically during cytokinesis allowing the new‐flagellum and the old‐flagellum daughters to be distinguished. We discovered that there are distinct morphological differences between the two daughters, with the new‐flagellum daughter in particular re‐modelling rapidly and extensively in early G1. This re‐modelling process involves an increase in cell body, flagellum and flagellum attachment zone length and is accompanied by architectural changes to the anterior cell end. The old‐flagellum daughter undergoes a different G1 re‐modelling, however, despite this there was no difference in G1 duration of their respective cell cycles. This work demonstrates that the two daughters of a proliferative division of T. brucei are non‐equivalent and enables more refined morphological analysis of mutant phenotypes. We suggest all proliferative divisions in T. brucei and related organisms will involve non‐equivalence.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 1.2MB, Terms of use)
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- Publisher copy:
- 10.1111/mmi.14345
Authors
- Publisher:
- Wiley
- Journal:
- Molecular Microbiology More from this journal
- Volume:
- 112
- Issue:
- 3
- Pages:
- 1024-1040
- Publication date:
- 2019-07-25
- Acceptance date:
- 2019-07-08
- DOI:
- EISSN:
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1365-2958
- ISSN:
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0950-382X
- Pmid:
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31286583
- Language:
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English
- Keywords:
- Pubs id:
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pubs:1032322
- UUID:
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uuid:f37176f2-9ad3-4b6a-9dda-5ea18859dc02
- Local pid:
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pubs:1032322
- Source identifiers:
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1032322
- Deposit date:
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2019-07-26
Terms of use
- Copyright holder:
- Abeywickrema, M et al.
- Copyright date:
- 2019
- Rights statement:
- © 2019 The Authors. Molecular Microbiology Published by John Wiley and Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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