Affordable in-house antiretroviral drug resistance assay with good performance in non-subtype B HIV-1.

Journal article

The introduction of antiretroviral (ARV) therapy in resource-poor settings is effective in suppressing HIV-1 replication and prolonging life of infected individuals. This has led to a demand for affordable HIV-1 drug resistance assays, since treatment failure due to development of drug resistance is common. This study developed and evaluated an affordable "in-house" genotyping assay to monitor HIV-1 drug resistance in Africa, particularly South Africa. An "in-house" assay using automated RNA ...

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Authors: Wallis, C; Papathanasopoulos, M; Lakhi, S; Karita, E; Kamali, A

• Publication status: Published
• Journal: Journal of virological methods
• Volume: 163
• Issue: 2
• Pages: 505-508
• Publication date: 2010-02-01
• DOI: 10.1016/j.jviromet.2009.11.011
• EISSN: 1879-0984
• ISSN: 0166-0934
• Language: English
• Keywords: Automation; Anti-Retroviral Agents; Humans; Sensitivity and Specificity; Genes, Viral; RNA, Viral; South Africa; Genotype; Microbial Sensitivity Tests; HIV-1; DNA Primers; Drug Resistance, Viral; Reverse Transcriptase Polymerase Chain Reaction