- Abstract:
-
Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases associated with one transcription unit. To count engaged polymerases, cells were encapsulated in agarose, permeabilized, treated with ribonuclease, and the now-truncated transcripts extended in [32P]uridine triphosphate; then, the number of growing transcripts was calculated from the total number of nucl...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1091/mbc.9.6.1523
-
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- Journal:
- Molecular biology of the cell
- Volume:
- 9
- Issue:
- 6
- Pages:
- 1523-1536
- Publication date:
- 1998-06-05
- DOI:
- EISSN:
-
1939-4586
- ISSN:
-
1059-1524
- URN:
-
uuid:f33d85de-1356-4d72-aca4-c95bafa690ec
- Source identifiers:
-
19706
- Local pid:
- pubs:19706
- Copyright date:
- 1998
Journal article
Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei.
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