Limiting dilution analysis of helper T-cell function. II. An approach to the study of the function of single helper T cells.

Journal article

Procedures have been described which are suitable for experiments utilizing a microculture system whereby one can easily study the function of single helper T cells. These are: (a) the elimination of the background anti-hapten response by preliminary anti-theta + guinea-pig complement complement treatment of the 'hapten-primed' population; (b) the removal of any contaminating hapten-specific B cells from the carrier-primed population by passage through nylon wool; (c) irradiation of long-term primed 'nylon wool' enriched helper cells tp prevent cell division; (d) the partition of individual helper cells by limiting dilution.

Authors: Waldmann, H; Pope, H; Lefkovits, I

• Publication status: Published
• Journal: Immunology
• Volume: 31
• Issue: 3
• Pages: 343-352
• Publication date: 1976-09-01
• EISSN: 1365-2567
• ISSN: 0019-2805
• Language: English
• Keywords: Haptens; T-Lymphocytes; B-Lymphocytes; Mice; Antibody-Producing Cells; Hemolytic Plaque Technique; Dose-Response Relationship, Immunologic; Leukocyte Count; Animals