Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B.

Boyd, R; Adam, P; Patel, S; Loader, J; Berry, J; Redpath, N; Poyser, H; Fletcher, G; Burgess, N; Stamps, A; Hudson, L; Smith, P; Griffiths, M; Willis, T; Karran, E; Oscier, D; Catovsky, D; Terrett, J; Dyer, M

Journal article

Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B.

Abstract:: B-cell-specific plasma-membrane proteins are potential targets for either small molecule or antibody-based therapies. We have sought to annotate proteins expressed at the cell surface membrane in patients with chronic lymphocytic leukemia (CLL) using plasma-membrane-based proteomic analysis to identify previously uncharacterized and potentially B-cell-specific proteins. Proteins from plasma-membrane fractions were separated on one-dimensional gels and trypsinized fractions subjected to high-throughput MALDI-TOF mass spectrometry. Using this method, many known B-cell surface antigens were detected, but also known proteins not previously described in this disease or in this cellular compartment, including cell surface receptors, membrane-associated enzymes and secreted proteins, and completely unknown proteins. To validate the method, we show that BLK, a B-cell-specific kinase, is located in the CLL-plasma-membrane fraction. We also describe two novel proteins (MIG2B and B-cell novel protein #1, BCNP1), which are expressed preferentially in B cells. MIG2B is in a highly conserved and defined gene family containing two plasma-membrane-binding ezrin/radixin/moesin domains and a pleckstrin homology domain; the Caenorhabditis elegans homolog (UNC-112) is a membrane-associated protein that colocalizes with integrin at cell-matrix adhesion complexes. BCNP1 is a completely unknown protein with three predicted transmembrane domains, with three alternatively spliced final exons. Proteomic analysis may thus define new potential therapeutic targets.

Publication status:: Published

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APA Style

Boyd, R., Adam, P., Patel, S., Loader, J., Berry, J., Redpath, N., Poyser, H., Fletcher, G., Burgess, N., Stamps, A., Hudson, L., Smith, P., Griffiths, M., Willis, T., Karran, E., Oscier, D., Catovsky, D., Terrett, J., & Dyer, M. (2003). Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B. Leukemia, 17(8), 1605–1612.

MLA Style

Boyd, R, et al. “Proteomic Analysis of the Cell-Surface Membrane in Chronic Lymphocytic Leukemia: Identification of Two Novel Proteins, BCNP1 and MIG2B.” Leukemia, vol. 17, no. 8, 2003, pp. 1605–12.

Chicago Style

Boyd, R, P Adam, S Patel, et al. 2003. “Proteomic Analysis of the Cell-Surface Membrane in Chronic Lymphocytic Leukemia: Identification of Two Novel Proteins, BCNP1 and MIG2B.” Leukemia 17 (8): 1605–12.
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