Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B.

Journal article

B-cell-specific plasma-membrane proteins are potential targets for either small molecule or antibody-based therapies. We have sought to annotate proteins expressed at the cell surface membrane in patients with chronic lymphocytic leukemia (CLL) using plasma-membrane-based proteomic analysis to identify previously uncharacterized and potentially B-cell-specific proteins. Proteins from plasma-membrane fractions were separated on one-dimensional gels and trypsinized fractions subjected to high-t...

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Authors: Boyd, R; Adam, P; Patel, S; Loader, J; Berry, J

• Publication status: Published
• Journal: Leukemia
• Volume: 17
• Issue: 8
• Pages: 1605-1612
• Publication date: 2003-08-01
• DOI: 10.1038/sj.leu.2402993
• EISSN: 1476-5551
• ISSN: 0887-6924
• Language: English
• Keywords: Blotting, Western; Leukemia, Lymphocytic, Chronic, B-Cell; Open Reading Frames; Neoplasm Proteins; Protein Structure, Tertiary; Humans; B-Lymphocytes; Protein Isoforms; Proteomics; Molecular Sequence Data; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Membrane Proteins; Base Sequence