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Recombinant Plasmodium vivax circumsporozoite surface protein allelic variants: antibody recognition by individuals from three communities in the Brazilian Amazon

Abstract:
Circumsporozoite protein (CSP) variants of P. vivax, besides having variations in the protein repetitive portion, can differ from each other in aspects such as geographical distribution, intensity of transmission, vectorial competence and immune response. Such aspects must be considered to P. vivax vaccine development. Therefore, we evaluated the immunogenicity of novel recombinant proteins corresponding to each of the three P. vivax allelic variants (VK210, VK247 and P. vivax-like) and of the C-terminal region (shared by all PvCSP variants) in naturally malaria-exposed populations of Brazilian Amazon. Our results demonstrated that PvCSP-VK210 was the major target of humoral immune response in studied population, presenting higher frequency and magnitude of IgG response. The IgG subclass profile showed a prevalence of cytophilic antibodies (IgG1 and IgG3), that seem to have an essential role in protective immune response. Differently of PvCSP allelic variants, antibodies elicited against C-terminal region of protein did not correlate with epidemiological parameters, bringing additional evidence that humoral response against this protein region is not essential to protective immunity. Taken together, these findings increase the knowledge on serological response to distinct PvCSP allelic variants and may contribute to the development of a global and effective P. vivax vaccine..
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41598-020-70893-3

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Role:
Author
ORCID:
0000-0002-9772-849X


Publisher:
Nature Research
Journal:
Scientific reports More from this journal
Volume:
10
Article number:
14020
Publication date:
2020-08-20
Acceptance date:
2020-08-02
DOI:
EISSN:
2045-2322
ISSN:
2045-2322
Pmid:
32820195


Language:
English
Keywords:
Pubs id:
1127682
Local pid:
pubs:1127682
Deposit date:
2020-08-27

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