Arabidopsis Rab-E GTPases exhibit a novel interaction with a plasma-membrane phosphatidylinositol-4-phosphate 5-kinase.

Journal article

Rab GTPases of the Arabidopsis Rab-E subclass are related to mammalian Rab8 and are implicated in membrane trafficking from the Golgi to the plasma membrane. Using a yeast two-hybrid assay, Arabidopsis phosphatidylinositol-4-phosphate 5-kinase 2 (PtdIns(4)P 5-kinase 2; also known as PIP5K2), was shown to interact with all five members of the Rab-E subclass but not with other Rab subclasses residing at the Golgi or trans-Golgi network. Interactions in yeast and in vitro were strongest with RAB...

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Authors: Camacho, L; Smertenko, A; Pérez-Gómez, J; Hussey, P; Moore, I

• Publication status: Published
• Journal: Journal of cell science
• Volume: 122
• Issue: Pt 23
• Pages: 4383-4392
• Publication date: 2009-12-01
• DOI: 10.1242/jcs.053488
• EISSN: 1477-9137
• ISSN: 0021-9533
• Language: English
• Keywords: Cell Membrane; Two-Hybrid System Techniques; Protein Binding; Plants, Genetically Modified; Arabidopsis Proteins; Point Mutation; Phosphotransferases (Alcohol Group Acceptor); Plant Leaves; rab GTP-Binding Proteins; Tobacco