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Interaction of quantitative PCR components with polymeric surfaces.

Abstract:

This study investigated the effect of exposing a polymerase chain reaction (PCR) mixture to capillary tubing of different materials and lengths, at different contact times and flow rates and the adsorption of major reaction components into the tubing wall. Using 0.5 mm ID tubing, lengths of 40 cm and residence times up to 45 min, none of the tested polymeric materials was found to affect subsequent PCR amplification. However, after exposure of the mixture to tubing lengths of 3 m or reduction...

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Publisher copy:
10.1007/s10544-006-9030-6

Authors


Gonzalez, A More by this author
Journal:
Biomedical microdevices
Volume:
9
Issue:
2
Pages:
261-266
Publication date:
2007-04-05
DOI:
EISSN:
1572-8781
ISSN:
1387-2176
URN:
uuid:f2851c1e-b350-4f30-bdee-44a4e3ef9fed
Source identifiers:
369428
Local pid:
pubs:369428

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