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A reference map of human lung MRC-5 fibroblast proteins using immobilized pH gradient-isoelectric focusing-based two-dimensional electrophoresis.

Abstract:

We report the first protein map of human adult lung MRC-5 fibroblasts using isoelectric focusing-immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis. MRC-5 is an immortalised cell line used in a wide range of investigations. The two-dimensional gel pattern of proteins generated from any given cell system provides a fingerprint that is unique to those cells. Therefore, the establishment of a protein map for a particular cell system provides a useful reference tool ...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Welson, SY More by this author
Abraham, DJ More by this author
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Journal:
Proteomics
Volume:
1
Issue:
6
Pages:
787-794
Publication date:
2001-06-05
DOI:
EISSN:
1615-9861
ISSN:
1615-9853
URN:
uuid:f2717b59-f99b-4eca-921b-fd67121bd9e9
Source identifiers:
230084
Local pid:
pubs:230084

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