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Secretion of recombinant human IgE-Fc by mammalian cells and biological activity of glycosylation site mutants.

Abstract:

We have constructed an expression vector that leads to secretion of the whole Fc of human immunoglobulin E (hIgE-Fc) from mammalian cells at levels up to 100 mg/l of culture. Two surface glycosylation sites at Asn265 and Asn371 have been changed to glutamine, to obtain a more homogeneous preparation of hIgE-Fc for structural studies. Comparison of wild-type and mutant products revealed that Asn371 is rarely glycosylated in Chinese hamster ovary cells. Both the double mutant and wild-type hIgE...

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Publication status:
Published

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Publisher copy:
10.1093/protein/8.2.193

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Mackay, GA More by this author
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Journal:
Protein engineering
Volume:
8
Issue:
2
Pages:
193-199
Publication date:
1995-02-05
DOI:
EISSN:
1460-213X
ISSN:
0269-2139
URN:
uuid:f25057bd-9284-48d3-970d-c09c7630a63b
Source identifiers:
171609
Local pid:
pubs:171609

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