Journal article
Unambiguous phosphosite localization using electron-transfer/higher-energy collision dissociation (EThcD).
- Abstract:
-
We recently introduced a novel scheme combining electron-transfer and higher-energy collision dissociation (termed EThcD), for improved peptide ion fragmentation and identification. We reasoned that phosphosite localization, one of the major hurdles in high-throughput phosphoproteomics, could also highly benefit from the generation of such EThcD spectra. Here, we systematically assessed the impact on phosphosite localization utilizing EThcD in comparison to methods employing either ETD or HCD...
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- Publication status:
- Published
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- Publisher copy:
- 10.1021/pr301130k
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Authors
Bibliographic Details
- Journal:
- Journal of proteome research
- Volume:
- 12
- Issue:
- 3
- Pages:
- 1520-1525
- Publication date:
- 2013-03-01
- DOI:
- EISSN:
-
1535-3907
- ISSN:
-
1535-3893
- Source identifiers:
-
384543
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:384543
- UUID:
-
uuid:f24e9dc4-5e97-411f-b802-511219e62db9
- Local pid:
- pubs:384543
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2013
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