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Journal article

Competitive binding to the oligopeptide binding protein, OppA: in-trap cleanup in an Fourier transform ion cyclotron resonance mass spectrometer.

Abstract:

This communication demonstrates that gentle infrared laser heating can remove unwanted buffer adducts from a gas-phase protein complex without dissociating the complex itself. Specifically, noncovalent complexes of the oligopeptide-binding protein, OppA, bound to either (Ala)3 or LysTrpLys were electrosprayed from aqueous buffer solution into a 9.4 tesla Fourier transform ion cyclotron resonance mass spectrometer. In addition to the intact complexes, several additional buffer adduct species w...

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Publication status:
Published

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Journal:
Journal of the American Society for Mass Spectrometry
Volume:
11
Issue:
11
Pages:
1023-1026
Publication date:
2000-11-05
DOI:
EISSN:
1879-1123
ISSN:
1044-0305
URN:
uuid:f24d6a0a-e2ee-4e46-b4ac-2cb8fcef0da2
Source identifiers:
59374
Local pid:
pubs:59374

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