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Journal article

Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells.

Abstract:

The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells using microfluidic arrays. As part of a study exploring the effect of genotype on Wnt pathway activation, data were collected for 96 qPCR assays on 1440 lymphoblastoid cells. The description of meth...

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Publication status:
Published

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Publisher copy:
10.1016/j.ymeth.2012.10.004

Authors


Tipping, AJ More by this author
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Journal:
Methods (San Diego, Calif.)
Volume:
59
Issue:
1
Pages:
71-79
Publication date:
2013-01-05
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
URN:
uuid:f215590d-d38d-4e75-8696-6a41bfdc8fd8
Source identifiers:
359730
Local pid:
pubs:359730

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