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Ion mobility-mass spectrometry analysis of large protein complexes.

Abstract:

Here we describe a detailed protocol for both data collection and interpretation with respect to ion mobility-mass spectrometry analysis of large protein assemblies. Ion mobility is a technique that can separate gaseous ions based on their size and shape. Specifically, within this protocol, we cover general approaches to data interpretation, methods of predicting whether specific model structures for a given protein assembly can be separated by ion mobility, and generalized strategies for dat...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2008.78
Journal:
Nature protocols More from this journal
Volume:
3
Issue:
7
Pages:
1139-1152
Publication date:
2008-01-01
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Language:
English
Keywords:
Pubs id:
pubs:59315
UUID:
uuid:f1949b81-4ec9-4cf0-b7d6-858335f183e5
Local pid:
pubs:59315
Source identifiers:
59315
Deposit date:
2012-12-19

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