The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance.

Journal article

The kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distance from the velocity, thus yielding a relationship between this distance and the binding rate. Numerical values of the binding site densities allowed absolute determination of the rate of association between two individual molecules as a function of the distance between attachment points. In our model, this rate was about 0.03 s-1 at 10 nm separation, and it was inversely proportional to the cube of the distance.

Authors: Pierres, A; Benoliel, A; Bongrand, P; Van Der Merwe, P

• Journal: FEBS letters
• Volume: 403
• Issue: 3
• Pages: 239-244
• Publication date: 1997-02-01
• DOI: 10.1016/s0014-5793(97)00060-4
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: Protein Binding; Microspheres; Ligands; Antigens, CD; Animals; Kinetics; Cell Adhesion Molecules; Antigens, CD2; Rats