Development of a new bimodal imaging methodology: a combination of fluorescence microscopy and high-resolution secondary ion mass spectrometry.

Journal article

In this paper, we present a new experimental methodology to combine mass spectrometry (NanoSIMS) with fluorescence microscopy to provide subcellular information on the location of small molecules in cultured cells. We demonstrate this by comparing the distribution of 5-bromo-2-deoxyuridine in the same cells given by both NanoSIMS analysis and by fluorescence immunohistochemistry. Fiducial markers in the substrates ensured that the images formed by SIMS mapping of bromine ions could be co-regi...

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Authors: Lau, K; Christlieb, M; Schröder, M; Sheldon, H; Harris, A

• Publication status: Published
• Journal: Journal of microscopy
• Volume: 240
• Issue: 1
• Pages: 21-31
• Publication date: 2010-10-01
• DOI: 10.1111/j.1365-2818.2010.03380.x
• EISSN: 1365-2818
• ISSN: 0022-2720
• Language: English
• Keywords: Fluorescent Antibody Technique; Cells, Cultured; Hela Cells; Humans; Microscopy, Fluorescence; Spectrometry, Mass, Secondary Ion; Bromodeoxyuridine; Diagnostic Imaging; Molybdenum; Umbilical Cord; Endothelial Cells