Development of a refined tenocyte differentiation culture technique for tendon tissue engineering.

Journal article

We have established that human tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that ...

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Authors: Qiu, Y; Wang, X; Zhang, Y; Carr, A; Zhu, L

• Publication status: Published
• Journal: Cells, tissues, organs
• Volume: 197
• Issue: 1
• Pages: 27-36
• Publication date: 2013-01-01
• DOI: 10.1159/000341426
• EISSN: 1422-6421
• ISSN: 1422-6405
• Language: English
• Keywords: Cell Growth Processes; Humans; Cell Survival; Adolescent; Cell Culture Techniques; Culture Media; Coloring Agents; Staining and Labeling; Cattle; Cells, Cultured; Tendons; Male; Animals; Tissue Engineering; Cell Differentiation; Azo Compounds; Young Adult; Adult; Collagen