Quantification of 32P-labeled samples in gel fragments using the flat-bed liquid scintillation counter.

Journal article

Quantification of 32P in bands after gel electrophoresis was performed using the flat-bed scintillation counter (Betaplate). The most convenient system involved placing fragments of dried gel between two glass fiber sheets, each previously sealed in a thin plastic bag with liquid scintillant. Good pulse-height spectra and counting efficiencies were obtained with low cross talk and background. The method has been used to quantify mRNA in RNA antisense-protection assays that were linear over a wide range (1-20000 cpm). Cross talk and background could be reduced further by an alternative technique utilizing plastic trays with shallow wells in which a solid scintillant had been melted. Fragments were immersed in the molten scintillant (90 degrees C), which was allowed to solidify, by cooling, before counting.

Authors: Potter, C; Tan, C; Ratcliffe, P

• Publication status: Published
• Journal: Analytical biochemistry
• Volume: 197
• Issue: 1
• Pages: 121-124
• Publication date: 1991-08-01
• DOI: 10.1016/0003-2697(91)90366-2
• EISSN: 1096-0309
• ISSN: 0003-2697
• Language: English
• Keywords: Anoxia; Electrophoresis, Polyacrylamide Gel; Animals; Rats; RNA, Messenger; Scintillation Counting; Electrophoresis, Agar Gel; Phosphorus Radioisotopes; Erythropoietin; Kidney