The effect of sample degradation and RNA stabilization on classical swine fever virus RT-PCR and ELISA methods.

Journal article

Classical swine fever (CSF), also known as hog cholera, is a highly contagious viral infection of swine caused by a member of the genus pestivirus of the family, Flaviviridae. The need for accurate laboratory diagnosis of CSF is particularly important as it is more reliable than clinical diagnosis. CSF is endemic in many tropical countries where the climate is characterized by high ambient temperature and humidity. This study details the effect of sample quality on CSF antigen-capture ELISA (...

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Authors: Blacksell, S; Khounsy, S; Westbury, H

• Publication status: Published
• Journal: Journal of virological methods
• Volume: 118
• Issue: 1
• Pages: 33-37
• Publication date: 2004-06-01
• DOI: 10.1016/j.jviromet.2004.01.015
• EISSN: 1879-0984
• ISSN: 0166-0934
• Language: English
• Keywords: DNA, Viral; Classical Swine Fever; Virology; Classical swine fever virus; DNA Primers; Reverse Transcriptase Polymerase Chain Reaction; Swine; Base Sequence; Enzyme-Linked Immunosorbent Assay; RNA Stability; RNA, Viral; Animals