Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes.

Journal article

Previous analyses of the uteroglobin gene promoter revealed a GT1 box which is also found in the SV40 enhancer. The GT1 element in the context of the uteroglobin promoter is active in Ishikawa cells, a human endometrial cell line, but not in HeLa cells. Here we report the cloning by recognition site screening of two factors (SPR-1 and SPR-2) which bind to this GT1 motif. SPR-1 and SPR-2 are homologues of the transcription factor Sp1. All three proteins are closely related members of a gene family encoding proteins with very similar structural features. Like Sp1, SPR-1 and SPR-2 contain glutamine and serine/threonine rich amino acid stretches. Most significantly, the DNA binding domains of all three proteins are highly conserved and they recognize GT as well as GC boxes identically. SPR-2 mRNA is expressed ubiquitously, whereas SPR-1 transcripts are abundant in the brain but barely detectable in other organs. The possible function of these factors for the activity of the uteroglobin promoter is discussed.

Authors: Hagen, G; Müller, S; Beato, M; Suske, G

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 20
• Issue: 21
• Pages: 5519-5525
• Publication date: 1992-11-01
• DOI: 10.1093/nar/20.21.5519
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Base Sequence; Sequence Homology, Amino Acid; Rabbits; Multigene Family; DNA-Binding Proteins; Cell Line; Mice; Binding Sites; Sp3 Transcription Factor; Promoter Regions, Genetic; Uteroglobin; Sp1 Transcription Factor; Cloning, Molecular; Animals; Brain; Blotting, Northern; Amino Acid Sequence; Humans; DNA; Molecular Sequence Data; Organ Specificity; Sp4 Transcription Factor; Transcription, Genetic; Polymerase Chain Reaction