Purification and characterization of endogenous peptides extracted from HLA-DR isolated from the spleen of a patient with rheumatoid arthritis.

Journal article

We have purified HLA-DR from the spleen of a patient with rheumatoid arthritis. The patient had Felty's syndrome and was heterozygous for the DR4Dw4 antigen. We have isolated endogenous peptides from purified HLA-DR molecules. The peptides were purified by reverse phase HPLC and the major peaks were subjected to N-terminal sequencing. The peptides were derived from a variety of proteins: human serum albumin, human erythroid protein 4.1, 60S ribosomal proteins L31 and L35, VCAM-1, human immuno...

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Authors: Gordon, R; Young, J; Rayner, S; Luke, R; Crowther, M

• Publication status: Published
• Journal: European journal of immunology
• Volume: 25
• Issue: 5
• Pages: 1473-1476
• Publication date: 1995-05-01
• DOI: 10.1002/eji.1830250553
• EISSN: 1521-4141
• ISSN: 0014-2980
• Language: English
• Keywords: Autoantigens; HLA-D Antigens; Neuropeptides; Cell Adhesion Molecules; Antigen Presentation; Humans; Cathepsins; Cytoskeletal Proteins; Ribosomal Proteins; Sequence Homology, Amino Acid; Epitopes; Amino Acid Sequence; Animals; Recombinant Fusion Proteins; Spodoptera; Vascular Cell Adhesion Molecule-1; Protein Binding; Arthritis, Rheumatoid; Membrane Proteins; Immunoglobulin lambda-Chains; Chromatography, High Pressure Liquid; Cell Line; Sequence Alignment; Peptide Fragments; Serum Albumin; Macromolecular Substances; Protein Structure, Tertiary; Autoimmune Diseases; Genetic Vectors; Molecular Sequence Data; Spleen; Nucleopolyhedrovirus